Sample Handling Makes the Difference: New Research on Tongue Tip Fluid Diagnostics

New research led by the University of Minnesota and supported by the Swine Health Information Center (SHIC) confirms that how tongue tip fluid samples are collected, processed, and stored directly impacts the accuracy of disease diagnostics, particularly for detecting PRRS.

Tongue tip sampling has emerged as a cost-effective and labor-efficient method for disease surveillance in swine herds. However, SHIC Associate Director Dr. Lisa Becton emphasizes that precision in sampling protocols is key to ensuring reliable results.

“The effectiveness can be affected by different sampling and handling methodologies,” says Dr. Becton.
“Pooling samples is more economical than individual testing, but it may slightly reduce diagnostic sensitivity. Quick freezing after collection preserves viral integrity and improves detection rates.”

Key Takeaways:

  • Pooling samples offers broader herd surveillance at a lower cost but with slightly reduced sensitivity.

  • Immediate freezing of samples after collection results in higher PRRS virus detection compared to refrigeration.

  • Time to cooling or freezing is critical to maintaining diagnostic accuracy.

The study reinforces the value of tongue tip fluids as a surveillance tool—especially when proper storage and processing procedures are followed. In a production setting, this insight provides practical guidance for balancing cost, efficiency, and diagnostic precision.

👉 Full study results available at: swinehealth.org